AlloSeq Tx17 | CareDx

This is superior HLA typing, period. Best-in-class solution to help you identify the best genetic match.

What is AlloSeq™ Tx 17?

AlloSeq Tx 17 is an innovative NGS HLA typing solution that uses Hybrid Capture Technology, which eliminates the inefficiencies and limitations of traditional Long-Range PCR methods.

AlloSeq Tx 17 goes beyond the traditional transplant related loci to consider more transplant associated genes and helps you to identify the best genetic match.

CareDx introduces AlloSeq Tx 17, the next generation in genetic matching, it is an innovative NGS HLA typing solution that uses hybrid capture technology, which eliminates the inefficiencies of using traditional long range PCR methods. AlloSeq Tx 17 goes beyond the traditional transplant related Loci to consider more transplant associated genes and helps you to identify the best genetic match. AlloSeq Tx 17 improve laboratory workflow provides multiple different advantages. Let’s look at each of these steps in detail. The first step of library generation is a four and one process where for each sample, well, genomic DNA is bound, fragmented, normalized and tagged by the bead bound transposons. This tagmentation is followed by the addition of index adapters, this process allows for less than 50 nanograms of input DNA. It then undergoes PCR amplification to create libraries that have unique indices and the whole genome data. This early indexing step leads to reduced risk of sample switching compared to long range PCR methods and does not require any manual quantification of DNA prior to pulling you then pull all of the sample libraries into a single tube for a single tube workflow. And also have the ability to add additional genes without impacting this workflow. The next step is hybrid capture. Let’s understand how our regions of interest are enriched in this process. First, we add a biotintulated probe panel to our pooled library. The biotintulated probe binds to the region of interest and is then enriched using striphaveton magnetic beads. The regions of interest are then alluded to produce enriched, targeted library. That are then ready for sequencing on alumina platforms. After 17 to 24 hours of sequencing, our proprietary AlloSeq Assign software helps to evaluate the results. The uniqueness of AlloSeq Assign lies in the fact that you don’t need any special hardware or software requirements to run it. It will show you the reeds as they are without any modification. And most important of all, it has a really fast data import speed. With the right settings, you may be able to get the import speed of one minute per sample, saving you valuable time with its innovative hybrid capture technology, AlloSeq Tx 17, offers advantages that long range picture does not, like the expandable gene content without affecting the lab workflow and easy single to workflow with early indexing. AlloSeq Tx 17, also offers improved allele balance compared to long range PCR techniques, wherein one allele is usually favored over the other. Another advantage with AlloSeq Tx 17, is reduced allele dropouts in long range PCR. Based on the primary design, Allele may or may not amplify, resulting in the drop out. However, with AlloSeq Tx 17 probes bind and capture regions of interest, including bases that did not bind PCR primers, resulting in no Allele dropout. Thus confirming that AlloSeq Tx 17 will provide better performance through improved allele imbalance, reduced dropout rates and no PCR artifacts or crossovers as compared to long range PCR techniques. And finally, the fast import speed of up to one minute per sample and enhanced user interface that will save time and effort proves AlloSeq Tx 17 is truly the next generation in genetic matching.

What are the benefits?

No long-range PCR inefficiencies-and no artifacts or crossovers
Improved allele balance and reduced dropout rates
Easy single tube workflow with early indexing and sample pooling
Expanded gene content enabling the highest level of transplant matching

Fast import speed and optimized data analysis with AlloSeq Assign^® software
Allele level resolution with low rates of ambiguity
Maximum coverage of 17 loci including all exons for all genes

How does it work?

A whole genome library is prepared using bead-bound transposons. Each sample is indexed and pooled together into a single tube. Genes of interest are captured with biotinylated probes and enriched for sequencing on an illumina system. Data is analyzed using AlloSeq Assign software.

Ordering details

Product	Product Number	Description
AlloSeq Tx 17 (RUO)	ASTX17.1(24)	Includes all reagents required to make 24 NGS libraries
AlloSeq Tx 17 (CE)	ASTX17.1(24)-IVD	Includes all reagents required to make 24 NGS libraries

Product	Product Number
AlloSeq Tx 17 (RUO) Includes all reagents required to make 24 NGS libraries	ASTX17.1(24)
AlloSeq Tx 17 (CE) Includes all reagents required to make 24 NGS libraries	ASTX17.1(24)-IVD

AlloSeq Tx17 is CE/IVD in European Union. For research use only in USA

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