AlloSeq Tx offers a single tube workflow and no long-range PCR inefficacies. Step 1 is Whole Genome Library Generation. Total time is 3.5 hours, 2 hours of hands-on time. Starting with tagmentation, genomic DNA is bound, fragmented and tagged by the bead bound transponsons – one sample per well. Less input data is required with less than 50ng of input DNA. Next, wash beads to remove any unbound DNA, index PCR amplification and clean up and size selection. Early indexing leads to reduced risk of sample switching. Next, pool all the sample libraries into a single tube. No manual qualification of DNA is required prior to pooling. This represents a single tube workflow. Additional genes can be added without impacting the workflow. Step 2 is hybrid capture. Total time is 5.5 hours, with 2 hours of hand-on time. First, hybridize probes for 90 min or overnight at 62 degrees Celsius, capture target balance, amplify, and clean up. No long-range PCR means you get consistent allele balance, reduced allele drop-out rates, and no PCR artifacts. Step 3 is sequencing on the Illumina platforms – MiSeq, MiniSeq, iSeq. Total time is 17 to 24 hours, with 15 minutes of hands-on time. Step 4 is software analysis. Total time is 20 minutes with 24 sample import using AlloSeq Assign. 1-minute sample import, as per internal testing and will depend on multiple factors. Fast data leads to fast analysis.