Every minute of laboratory time is valuable. AlloSeq® HCT can help you save time with a simple workflow and powerful analysis software, all while achieving high sensitivity chimerism monitoring. Engraftment monitoring (chimerism) testing evaluates the success of a hematopoietic stem cell transplant by measuring the relative ratio of the recipient and the donor cell population in the recipient’s post-transplant specimen. Engraftment monitoring is used in the diagnosis of disease relapse and graft failure.

What is AlloSeq HCT?

AlloSeq HCT is an NGS-based solution enabling precise measurement of engraftment following hematopoietic stem cell transplant for research applications

  • Simple, streamlined assay (one multiplexed reaction per sample)
  • Targets 202 bi-allelic SNPs across 22 autosomes
  • Validated for use on Illumina MiSeq

What are the benefits of AlloSeq HCT?

  • Streamlined workflow with minimal hands-on time (2 hrs)
  • Sample to result in less than 24 hours
  • Highly sensitive (0.3% limit of detection)
  • Accurate, precise, and reproducible results
  • User-friendly AlloSeq HCT software features automatic determination of informative loci, chimerism calculation, and results tracking
  • Able to determine up to 3 genomes in a single sample; useful for double cord blood transplants

Simple AlloSeq HCT workflow with minimal hands on

How does AlloSeq HCT work?

The AlloSeq HCT assay enables the amplification and sequencing of 202 single nucleotide polymorphisms (SNPs) across all autosomal chromosomes. The AlloSeq HCT software automatically calculates % recipient and donor DNA in post-transplant samples.

Rapid workflow with automated analysis

AlloSeq HCT Software provides easy and fast results with automated analysis

For research use only – not for use in diagnostic procedures