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Every minute of laboratory time is valuable. AlloSeq HCT can help you save time with a simple workflow and powerful analysis software, all while achieving high sensitivity chimerism monitoring.

Engraftment monitoring (chimerism) testing evaluates the success of a hematopoietic stem cell transplant by measuring the relative percentage of the recipient and the donor DNA in the recipient’s post-transplant specimen. Engraftment monitoring may assist in detecting disease relapse or graft failure.

What is AlloSeq HCT?

AlloSeq HCT is a targeted NGS-based solution enabling precise measurement of engraftment following hematopoietic stem cell transplant for research applications.

  • Sample to result in less than 24 hours
  • Test up to 48 samples/run
  • Targets 202 bi-allelic SNPs across 22 autosomes
  • Validated for use on Illumina MiSeq

What are the benefits of AlloSeq HCT?


  • 1 assay for genotyping and monitoring
  • 1-step single reaction multiplexed PCR
  • Highly sensitive – 0.22% limit of detection
  • Accurate, precise, and reproducible results
  • Streamlined workflow with minimal hands-on time within 3 hours

HCT Software

  • Able to determine and automatically compare up to 3 genotypes in a single sample – donor(s) and recipient: useful for cord blood transplants
  • User-friendly AlloSeq HCT software features automatic determination of informative loci, chimerism calculation, and result tracking

Simple AlloSeq HCT Workflow with Minimal Hands-On Time

How Does AlloSeq HCT Work?

The AlloSeq HCT assay enables the amplification and sequencing of 202 single nucleotide polymorphisms (SNPs) across all autosomal chromosomes. The AlloSeq HCT software automatically calculates % recipient and donor DNA in post-transplant samples.

Rapid Workflow with Automated Analysis

HOT: Hands-on time (based on 48 samples). TAT: Turn around time.

Perform Detailed Analysis with Just a Few Clicks

AlloSeq HCT is available as CE/IVD in the EU and UK, and Research Use Only for the rest of the world. For local regulatory status, please contact CareDx. AlloSeq is a trademark or registered trademark of CareDx Inc. or its subsidiaries in the US or other countries. AlloSeq is a registered trademark with the US Patent and Trademark Office.