Every minute of laboratory time is valuable. AlloSeq™ HCT can help you save time with a simple workflow and powerful analysis software, all while achieving high sensitivity chimerism monitoring. Engraftment monitoring (chimerism) testing evaluates the success of a hematopoietic stem cell transplant by measuring the relative ratio of the recipient and the donor cell population in the recipient’s post-transplant specimen. Engraftment monitoring is used in the diagnosis of disease relapse and graft failure.

What is AlloSeq HCT?

AlloSeq HCT is an NGS-based solution enabling precise measurement of engraftment following hematopoietic stem cell transplant for research applications

  • Simple, streamlined assay (one multiplexed reaction per sample)
  • Targets 202 bi-allelic SNPs across 22 autosomes
  • Validated for use on Illumina MiSeq

What are the benefits of AlloSeq HCT?

  • Streamlined workflow with minimal hands-on time (2 hrs)
  • Sample to result in less than 24 hours
  • Highly sensitive (0.3% limit of detection)
  • Accurate, precise, and reproducible results
  • User-friendly AlloSeq HCT software features automatic determination of informative loci, chimerism calculation, and results tracking
  • Able to determine up to 3 genomes in a single sample; useful for double cord blood transplants

Simple AlloSeq HCT workflow with minimal hands on

There is 13 minutes of DNA extraction time, 30 minutes of library prep time, followed by 17 hours of instrument run time, and finally less than 2 minutes of automated analysis, cumulating in less than 2 hours of total hands-on time and less than or equal to 24 hours of total assay time (including hands off time.)

How does AlloSeq HCT work?

The AlloSeq HCT assay enables the amplification and sequencing of 202 single nucleotide polymorphisms (SNPs) across all autosomal chromosomes. The AlloSeq HCT software automatically calculates % recipient and donor DNA in post-transplant samples.

Rapid workflow with automated analysis

Go from gDNA sample to report in less than twenty-four hours. Once DNA is extracted, amplification and sequencing begins. Step one includes PCR, one-point-five hours TAT and thirty minutes HOT. Step two includes POOL plus CLEAN, fifteen minutes TAT and fifteen minutes HOT. Step three includes QUANT plus LOAD, thirty minutes TAT and thirty minutes HOT. Sequencing, compatible with Illumina MiSeq, takes seventeen hours. Then transfer imports FASTQ files, analysis generates QC values and percentage gDNA, and reporting exports results to Excel and LIMS-compatible files.

AlloSeq HCT Software provides easy and fast results with automated analysis

Post-transplant samples create sample names, the analysis date, DNA type, sample quality, recipient DNA, donor-one DNA, donor-two DNA, and a details view that can be seen by clicking on it. Clicking on details view displays a detailed pop-up percentage of DNA for each sample. Recipient-only and donor-only results are also created with sample names, analysis date, DNA type, and sample quality. A details view display is also available for each of these.

For research use only – not for use in diagnostic procedures

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